in vitro developmental competence of bovine oocytes: effect of corpus luteum and follicle size
نویسندگان
چکیده
background: previous studies reported many discrepancies about the effects of corpus luteum (cl) and ovarian follicle size on the developmental competence of oocytes. objective: the aim of this study was to investigate the effects of cl and different size of follicle on the developmental potential of bovine oocytes. materials and methods: after ovarian classification based on presence or absence of cl, sample follicles were placed in three groups according to their diameter; small (s; 3–6 mm), medium (m; 6–9 mm), and large (l; 10–20 mm). collected oocytes in each group were subjected to the in vitro embryo production processes. results: results showed that, the percentages of blastocyst obtained from oocytes originating from small and medium follicles of ovaries bearing a cl (cl+s-oocytes and cl+m-oocytes, respectively) were lower (p<0.001) than those of small and medium follicles of ovaries not bearing a cl (cl-s-oocytes and cl-m-oocytes, respectively) (30.8% and 33.6% vs. 36.9% and 38.7% respectively). although, the percentages of blastocyst obtained from cl-m-oocytes and cl-l-oocytes were greater (p< 0.001) than those of cl+s-oocytes and cl+m-oocytes. there were no significant differences in the percentages of blastocyst formation between controls (c-oocytes), cl-s-oocytes and cl+l-oocytes. conclusion: according to the results of this study, the negative effect of cl on the developmental competence of bovine oocyte depends on the follicle size. therefore, oocytes originating from large grown follicles were not influenced by negative effects of cl as much as those originating from small and medium follicles did.
منابع مشابه
In vitro developmental competence of bovine oocytes: Effect of corpus luteum and follicle size.
BACKGROUND Previous studies reported many discrepancies about the effects of corpus luteum (CL) and ovarian follicle size on the developmental competence of oocytes. OBJECTIVE The aim of this study was to investigate the effects of CL and different size of follicle on the developmental potential of bovine oocytes. MATERIALS AND METHODS After ovarian classification based on presence or absen...
متن کاملin vitro developmental competence of bovine oocytes in relation to side of ovaries, presence of corpus luteum and follicle size
0
متن کاملthe effect of e-64 on the developmental competence of sheep cocs during in vitro maturation
in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.
: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
Effect of Culture System on Developmental Competence, Cryosurviva and DNA-Fragmentation of In Vitro Bovine Blastocysts
متن کامل
developmental competence of bovine oocytes selected based on follicle size and using the brilliant cresyl blue (bcb) test
background: many studies reported that follicle size has an essential role in developmental potential of oocytes. also, the brilliant cresyl blue (bcb) test is one of the most important criteria in selection of more competent oocytes. objective: selection of developmentally competent bovine oocytes. materials and methods: a total of 1730 bovine cumulus oocyte complexes (cocs) were recovered fro...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
international journal of reproductive biomedicineجلد ۱۳، شماره ۱۰، صفحات ۶۱۵-۰
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023